Difference between revisions of "Part:BBa K1741008"
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Xylose induced promoter xylA is weaker than arabinose and  rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.
 
Xylose induced promoter xylA is weaker than arabinose and  rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.
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===Characteristics and Results===
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https://static.igem.org/mediawiki/parts/0/0a/Teamuampoznanxyloseod600graph.png
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https://static.igem.org/mediawiki/parts/f/f8/Teamuampoznanxyloselbgraph.png
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https://static.igem.org/mediawiki/parts/5/5d/Teamuampoznanxylosem9graph.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:56, 27 September 2015

sfGFP under promoter xylA1 with improved 5'UTR

Xylose induced promoter xylA is weaker than arabinose and rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.

Characteristics and Results

Teamuampoznanxyloseod600graph.png

Teamuampoznanxyloselbgraph.png

Teamuampoznanxylosem9graph.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 419