Difference between revisions of "Part:BBa K1741008"

(Created page with "Xylose induced promoter xylA is weaker than arabinose and rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR fro...")
 
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__NOTOC__
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<partinfo>BBa_K1741008 short</partinfo>
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Xylose induced promoter xylA is weaker than arabinose and  rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.
 
Xylose induced promoter xylA is weaker than arabinose and  rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1741008 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1741008 parameters</partinfo>
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Revision as of 01:28, 18 September 2015

sfGFP under promoter xylA1 with improved 5'UTR

Xylose induced promoter xylA is weaker than arabinose and rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5’UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 419