Difference between revisions of "Part:BBa K1645999"

Revision as of 03:20, 26 September 2015

Dual Color Plasmid (RFP and GFP)

This part is a composite of the full GFP cassette (taken from registry part BBa_I20260) and the full RFP cassette (taken from registry part BBa_J04450). The GFP is under the constitutive J23101 promoter. The RFP is under the lacI (R0010) promoter, which is constitutive in DH5-alpha because lacI is not expressed. RFP expression is inhibited if lacI is present in the cell or if IPTG is not present. This part allowed us to visualise colour swaps caused by different SgRNA targeting the promoter region of the two colours colours.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1708
Illegal AgeI site found at 1820
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 706

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 <partinfo>BBa_K1645999 short</partinfo>
-This part is the composite part of GFP full cassette taken from registry part BBa_I20260 and RFP full cassette taken from registry part BBa_J04450. The GFP is under the J23101 promoter, which is a constitutive promoter. In addition, the RFP is under the lacI (R0010) promoter which is constitutive in DH5-alpha due to the absence of lacI expression. If lacI repressor is presented in the cell the RFP expression is inhibited and IPTG is needed for the expression of RFP. For our purposes, this part was used to see the colour swaps using different SgRNA targeting promoter region of each of the colours.
+This part is a composite of the full GFP cassette (taken from registry part BBa_I20260) and the full RFP cassette (taken from registry part BBa_J04450). The GFP is under the constitutive J23101 promoter. The RFP is under the lacI (R0010) promoter, which is constitutive in DH5-alpha because lacI is not expressed. RFP expression is inhibited if lacI is present in the cell or if IPTG is not present. This part allowed us to visualise colour swaps caused by different SgRNA targeting the promoter region of the two colours colours.
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