Difference between revisions of "Part:BBa K1692023"

Revision as of 06:15, 22 September 2015

Ptet + Luxl

Overview

Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet(BBa_R0040) and luxl generator (BBa_K805016). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 717
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1692023 short</partinfo>
-N/A
+<h2> Overview </h2>
+Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]) and luxl generator ([https://parts.igem.org/Part:BBa_K805016 BBa_K805016]). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct.
 <!-- Add more about the biology of this part here