Difference between revisions of "Part:BBa K1806005"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1806005 short</partinfo> | <partinfo>BBa_K1806005 short</partinfo> | ||
The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS. | The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS. | ||
| + | |||
| + | == Cloning == | ||
| + | |||
| + | The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. Sample 7-6 yielded the accurate base pair length. | ||
| + | |||
| + | [[File:6005-1.png]] [[File:6005-2.png]] | ||
| + | |||
| + | [[File:6005-3.png]] [[File:6005-4.png]] | ||
| + | |||
| + | == Functional Assay == | ||
| + | |||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 00:38, 20 September 2015
T7+ ibPB RNA Thermometer + RFP
The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS.
Cloning
The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. Sample 7-6 yielded the accurate base pair length.
Functional Assay
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 720
Illegal AgeI site found at 832 - 1000COMPATIBLE WITH RFC[1000]