Difference between revisions of "Part:BBa K1766003"

Revision as of 00:12, 18 September 2015

OmpR controlled MicF

MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.

OmpR controlled micF

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

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 This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.
 [[File:OmpRmicF.png|200px|thumb|left|OmpR controlled micF]]
 <!-- Add more about the biology of this part here