Difference between revisions of "Part:BBa K1766003"
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This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system. | This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system. | ||
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| + | [[File:K1766003.png|200px|thumb|left|alt text]] | ||
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<partinfo>BBa_K1766003 parameters</partinfo> | <partinfo>BBa_K1766003 parameters</partinfo> | ||
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| + | '''References''' | ||
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| + | [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12 | ||
Revision as of 00:07, 18 September 2015
OmpR controlled MicF
MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].
This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.
File:K1766003.png
alt text
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12