Difference between revisions of "Part:BBa K1692002"
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[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|This is a <b>SDS PAGE gel</b> with purified PAL, FDC and UbiX protein. We ran a Mark 12 protein ladder to verify that our proteins were the correct molecular weight.]]<br><br> | [[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|This is a <b>SDS PAGE gel</b> with purified PAL, FDC and UbiX protein. We ran a Mark 12 protein ladder to verify that our proteins were the correct molecular weight.]]<br><br> | ||
− | [[File:SB2015_styrene_absorbance.png|thumbnail|center| | + | [[File:SB2015_styrene_absorbance.png|thumbnail|center|400px|<b>Styrene Absorbance</b>The absorbance spectrum of pure styrene (1 mM) measured on a Spectramax Pro spectrophotometer]]<br><br> |
− | [[File:SB2015_tCA_absorbance.png|thumbnail|center| | + | [[File:SB2015_tCA_absorbance.png|thumbnail|center|400px|<b>trans-Cinnamic Acid Absorbance</b>The absorbance spectrum of pure trans-Cinnamic Acid (1 mM) measured on a Spectramax Pro spectrophotometer]]<br><br> |
− | [[File:SB2015_FMN_absorbance.png|thumbnail| | + | [[File:SB2015_FMN_absorbance.png|thumbnail|center|400px|<b>Flavin Mononucleotide Absorbance</b>The absorbance spectrum of pure Flavin Mononucleotide (1 mM) measured on a Spectramax Pro spectrophotometer]]<br><br> |
<h2>Reference</h2> | <h2>Reference</h2> |
Revision as of 23:52, 17 September 2015
codon optimized FDC with T7 promoter and Flag Tag
Introduction
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
Background
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 233
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]