Difference between revisions of "Part:BBa K1741004"
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| − | Twice higher expression has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promer is still weaker than araBAD or rhaBAD, so can be a promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein. | + | Twice higher expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promer is still weaker than araBAD or rhaBAD, so can be a promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein. |
Revision as of 23:39, 17 September 2015
Twice higher expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promer is still weaker than araBAD or rhaBAD, so can be a promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.