Difference between revisions of "Part:BBa K1585212"
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| − | This is the translational unit of the 6-phospho-3-hexuloisomerase ( | + | This is the translational unit of the 6-phospho-3-hexuloisomerase (Phi) from ''Bacillus methanolicus'' MGA3. It is codon optimized for ''E. coli''. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]] | ||
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| + | Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction. | ||
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Latest revision as of 23:16, 18 September 2015
B0034.PHI
This is the translational unit of the 6-phospho-3-hexuloisomerase (Phi) from Bacillus methanolicus MGA3. It is codon optimized for E. coli.
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| Catalysed reaction by Phi D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate. |
Usage and Biology
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]