Difference between revisions of "Part:BBa K1587006"
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<center>[[File:TLSE cI.jpg]]</center> | <center>[[File:TLSE cI.jpg]]</center> | ||
<center>'''Figure 1: Genetic construct for pompC and lacI box regulated cI expression'''</center> | <center>'''Figure 1: Genetic construct for pompC and lacI box regulated cI expression'''</center> | ||
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+ | The aim of this construct was to create a circadian switch to alternate the expression of two sets of genes (Figure 2). This enables the production of one protein by night and another by day. | ||
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+ | <center>[[File:TLSE-Circadian.JPG]]</center> | ||
+ | <center>'''Figure 2: Circadian gene expression switch'''</center> | ||
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Revision as of 15:55, 18 September 2015
pompC and lacI box regulated cI expression
Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly we did not succeed in cloning the final construct with ho1 and pcyA (Figure 1).
![TLSE cI.jpg](/wiki/images/2/23/TLSE_cI.jpg)
The aim of this construct was to create a circadian switch to alternate the expression of two sets of genes (Figure 2). This enables the production of one protein by night and another by day.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]