Difference between revisions of "Part:BBa K1587007"
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<center>'''Figure 1: Genetic construct for formate production.'''</center> | <center>'''Figure 1: Genetic construct for formate production.'''</center> | ||
− | + | Formate is a simple organic acid produced with an E.coli strain. The initial substrate, glucose, is decomposed into pyruvate during glycolysis, and formate is naturally synthesized thanks to two key genes: | |
+ | - '''pflB''' coding for pyruvate formate lyase which catalyzes the cleavage of pyruvate into C1 and C2. This enzyme is sensitive to oxygen and is only active in microaerobic or anaerobic conditions, which is the case within our device. | ||
+ | - '''pflA''' coding for pyruvate formate lyase activase, which is directly linked with the pyruvate formate lyase, enabling its activation. | ||
<center>[[File:TLSE ForMetabo.jpg]]</center> | <center>[[File:TLSE ForMetabo.jpg]]</center> |
Revision as of 21:43, 17 September 2015
Formate production
Strong RBS (BBa_1006) and formate producing enzyme pyruvate formate-lyase 1 pflB and pyruvate formate-lyase 1-activating enzyme pflA.
Formate is a simple organic acid produced with an E.coli strain. The initial substrate, glucose, is decomposed into pyruvate during glycolysis, and formate is naturally synthesized thanks to two key genes: - pflB coding for pyruvate formate lyase which catalyzes the cleavage of pyruvate into C1 and C2. This enzyme is sensitive to oxygen and is only active in microaerobic or anaerobic conditions, which is the case within our device. - pflA coding for pyruvate formate lyase activase, which is directly linked with the pyruvate formate lyase, enabling its activation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 405
Illegal BamHI site found at 2807 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 514
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2995