Difference between revisions of "Part:BBa K1741012"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1741012 short</partinfo>
 
<partinfo>BBa_K1741012 short</partinfo>
 +
==Design==
 +
 +
proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains  on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters.
 +
 +
Legend:
 +
proC [[https://parts.igem.org/Part:BBa_K1741012  BBa_K1741012]]
 +
proC with a hairpin in 5'UTR [[https://parts.igem.org/Part:BBa_K1741013  BBa_K1741013]]
 +
proD [[https://parts.igem.org/Part:BBa_K1741014  BBa_K1741014]]
 +
 +
===Characteristics and Results===
 +
 +
Legend:
 +
proC [[https://parts.igem.org/Part:BBa_K1741012  BBa_K1741012]]
 +
proC with a hairpin in 5'UTR [[https://parts.igem.org/Part:BBa_K1741013  BBa_K1741013]]
 +
proD [[https://parts.igem.org/Part:BBa_K1741014  BBa_K1741014]]
 +
 +
https://static.igem.org/mediawiki/parts/3/35/Teamuamprocdiffsugarsgraph.png
 +
 +
https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png
 +
 +
https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
 +
 +
https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
  
−
proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains  on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future... Expression is higher than of inducible promoters
+
The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:32, 27 September 2015

sfGFP under proC promoter

Design

proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters.

Legend: proC [BBa_K1741012] proC with a hairpin in 5'UTR [BBa_K1741013] proD [BBa_K1741014]

Characteristics and Results

Legend: proC [BBa_K1741012] proC with a hairpin in 5'UTR [BBa_K1741013] proD [BBa_K1741014]

Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

UAMpoznanproChairpin.jpg

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 201