Difference between revisions of "Part:BBa K1585200"
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[[File:Aachen_Reaction_mdh.png]]
 
[[File:Aachen_Reaction_mdh.png]]
 +
 
CH<sub>3</sub>OH + NAD(+) → CH<sub>2</sub>O + NADH
 
CH<sub>3</sub>OH + NAD(+) → CH<sub>2</sub>O + NADH
  

Revision as of 21:39, 17 September 2015

Mdh2

Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NAD+ dependent alcohol dehydrogenase that converts methanol to formaldehyde.

Aachen Reaction mdh.png

CH3OH + NAD(+) → CH2O + NADH

Usage and Biology

The Characterization was done with RBS B0034 and is documented here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]