Difference between revisions of "Part:BBa K1835500"

Revision as of 22:58, 18 September 2015

Phi X 147 E Lysis Gene

The phi X 174 bacteriophage is a phage that infects E. coli. The You Lab here at Duke shared with us a strain of bacteria carrying a plasmid which contained the phi X 174 bacteriophage’s E protein, which induces lysis in bacteria. Lysis is caused by a transmembrane tunnel created by the E protein. It is believed that the transmembrane tunnel forms as a result of the E protein inhibiting peptidoglycan synthesis during cell growth.

One example of an application for this cell death gene is that employed by Lingchong You, et al. They designed a construct that contained the lysis gene as well as quorum sensing components, which caused populations oscillations over time. The gene circuit did not work as anticipated, but the oscillations were a product of cell suicide caused by the E lysis gene.

The figure below graphically displays the E lysis protein’s effect on cell growth. The uninduced sample grew approximately four times as well as the fully induced sample.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1835500 short</partinfo>
-Bacteriophage's lysis gene that inhibits the creation of new cell wall after cell division.
+The phi X 174 bacteriophage is a phage that infects E. coli. The You Lab here at Duke shared with us a strain of bacteria carrying a plasmid which contained the phi X 174 bacteriophage’s E protein, which induces lysis in bacteria. Lysis is caused by a transmembrane tunnel created by the E protein.  It is believed that the transmembrane tunnel forms as a result of the E protein inhibiting peptidoglycan synthesis during cell growth.
+One example of an application for this cell death gene is that employed by Lingchong You, et al. They designed a construct that contained the lysis gene as well as quorum sensing components, which caused populations oscillations over time.  The gene circuit did not work as anticipated, but the oscillations were a product of cell suicide caused by the E lysis gene.
+The figure below graphically displays the E lysis protein’s effect on cell growth. The uninduced sample grew approximately four times as well as the fully induced sample.
 <!-- Add more about the biology of this part here