Difference between revisions of "Part:BBa K1587008"
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<center>[[File:TLSE Circ3.jpg]]</center> | <center>[[File:TLSE Circ3.jpg]]</center> | ||
<center>'''Figure 1: Chimeric Red light receptor cph8 genetic construct'''</center> | <center>'''Figure 1: Chimeric Red light receptor cph8 genetic construct'''</center> | ||
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<center>[[File:TLSE CircSchem.jpg]]</center> | <center>[[File:TLSE CircSchem.jpg]]</center> | ||
<center>'''Figure 2: Chimeric Red light receptor cph8 (cph1-envZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of ompR'''</center> | <center>'''Figure 2: Chimeric Red light receptor cph8 (cph1-envZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of ompR'''</center> | ||
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+ | Phosphorylation of ompR by cph8 inhibits the expression of the genes regulated by promoter pompR. Since ompR and pompR are naturaly found in ''E.coli'', this biobrick enables a circadian expression of genes regulated by pompR in E. coli straine deleted for envZ. | ||
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Revision as of 21:10, 17 September 2015
Red light biosensor
Strong promoter BBa_J23119.Strong RBS BBa_B0030 Chimeric Red light receptor cph8 BBa_I15010.
![TLSE Circ3.jpg](/wiki/images/1/10/TLSE_Circ3.jpg)
![TLSE CircSchem.jpg](/wiki/images/0/0a/TLSE_CircSchem.jpg)
Phosphorylation of ompR by cph8 inhibits the expression of the genes regulated by promoter pompR. Since ompR and pompR are naturaly found in E.coli, this biobrick enables a circadian expression of genes regulated by pompR in E. coli straine deleted for envZ.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 414
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]