Difference between revisions of "Part:BBa K1766002"
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This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.  
 
This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.  
  
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[[File:Fusion protein micf gfp.jpg|200px]]
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This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. The fluorescent colonies showed the right sequence for the micF target-GFP fusion protein when sequensed using Sanger sequencing. No further characterisation was attempted due to lack of time.  
  
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This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.  
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[[File:Fusion protein micf gfp.jpg|200px]]
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Revision as of 20:18, 17 September 2015

MicF regulated GFP

This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.

This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. The fluorescent colonies showed the right sequence for the micF target-GFP fusion protein when sequensed using Sanger sequencing. No further characterisation was attempted due to lack of time.

Fusion protein micf gfp.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861

References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12