Difference between revisions of "Part:BBa K1766002"
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This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF. | This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF. | ||
| − | [[File: | + | [[File:Fusion protein micf gfp.jpg|200}x{200}px]] |
This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. | This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. | ||
Revision as of 20:13, 17 September 2015
MicF regulated GFP
This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.
This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 861
References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12