Difference between revisions of "Part:BBa K1766002"

Revision as of 20:00, 17 September 2015

MicF regulated GFP

This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be silensed in presence of micF.

This part was created a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 861

References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1766002 short</partinfo>
-This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be silensed in presence of micF.
+This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be silensed in presence of micF.
 This part was created a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.