Difference between revisions of "Part:BBa K1766004"
 
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The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed to depend on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce red fluorescent protein in high osmolarity condition when phosphorylated OmpR expresses RFP. In low osmolarity condition, it is supposed to produce green fluorescent protein as the micF binds to its target site, leading to GFP expression.
 
The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed to depend on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce red fluorescent protein in high osmolarity condition when phosphorylated OmpR expresses RFP. In low osmolarity condition, it is supposed to produce green fluorescent protein as the micF binds to its target site, leading to GFP expression.
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[[File:K1766004.png|700px|thumb|left|OmpR regulated RFP/GFP]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:57, 17 September 2015

OmpR controlled regulatory circuit producing RFP and GFP

This part is a combination of OmpR regulated RFP, micF regulated GFP (containing micF target) and micF RNA. The part was designed to achieve a highly sensitive on-off switch mechanism in the OmpR dependent regulatory system.

The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed to depend on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce red fluorescent protein in high osmolarity condition when phosphorylated OmpR expresses RFP. In low osmolarity condition, it is supposed to produce green fluorescent protein as the micF binds to its target site, leading to GFP expression.


OmpR regulated RFP/GFP






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 992
    Illegal NheI site found at 1015
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 689
    Illegal AgeI site found at 801
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1846