Difference between revisions of "Part:BBa K1741003"

Revision as of 04:44, 27 September 2015

sfGFP under melibiose promoter

MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.

Results

Legend:
MelS BBa_K1741004
MelWT BBa_K1741003

picture picture picture

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 154
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 206

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1741003 short</partinfo>
 MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.
+===Results===
+<br>
+Legend:<br>
+MelS BBa_K1741004
+<br>
+MelWT BBa_K1741003
+picture https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
+picture https://static.igem.org/mediawiki/parts/2/2c/Teamuampoznanmelibioselbgraph.png
+picture https://static.igem.org/mediawiki/parts/a/a2/Teamuampoznandifferentpromotersm9minimal.png
 <!-- Add more about the biology of this part here