Difference between revisions of "Part:BBa K1602017"
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         <h1>Xylose to xylitol converting construct</h1>
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         <h1><small>D</small>-xylonic acid producing operon</h1>
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        <b>Xylose</b> is a monosaccharide belonging to the aldopentose family. Through reduction it can be converted to xylitol. The reaction takes place in the cytosol of the host and recent studies show, that the formation of xylitol in E.coli seems possible as well.  
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        <b><small>D</small>-Xylose</b> is a monosaccharide belonging to the aldopentose family. It was recently    shown that the <small>D</small>-xylose dehydrogenase <i>xylB</i> from <i>Caulobacter crescentus</i> can convert <small>D</small>-xylose to <small>D</small>-xylonolactone. This can react spontaneously or through the catalysation of <i>xylC</i> to <small>D</small>-xylonic acid. In <i>E. coli</i> <small>D</small>-xylonic acid can be further metabolized to ethyleneglycol.
 
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         <br>
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         To enable the reduction in <i>E.coli</i> it is mandatory to establish an operon containing the coding gene <i>GRE3</i> for a aldose reductase. The gene is taken from <i>Saccharomyces cerevisiae</i>. The aldose reductase converts xylose to xylitol in dependence of NADPH.
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         <img style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/Conversion_xylose_xylitol_v1.png"></div>
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         <img style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/Conversion_ xylose_xylitol_v1.png"></div>
 
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             <b>Figure 1</b> Reaction scheme of the xylose to xylitol converting operon. Xylose is the only substrate needed for the reaction. Xylose is metabolized to xylitol in 1 step in dependance of NADPH.
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             <b>Figure 1</b>  
 
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===<h2>Usage</h2>===
 
===<h2>Usage</h2>===
 
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             This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) in front of it, under control of a T7 Promoter (<a href="/Part:BBa_I719005">BBa_I719005</a>).
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             This part is a composite of two coding genes with strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>). The transcription is controlled by a T7 promotor (<a href="/Part:BBa_I719005">BBa_I719005</a>).
 
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                     <ul>
 
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                        <li class="block-10vi">aldose reductase - GRE3                     <a href="/Part:BBa_K1602004">(BBa_K1602004)</a></li>
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                    <li class="block-10vi">T7-promotor                      
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                    <a href="/Part:BBa_I719005">(BBa_I719005)</a></li>
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                    <li class="block-10vi">ribosome binding site B0034                    
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                    <a href="/Part:BBa_B0034">(BBa_B0034)</a></li>
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                    <li class="block-10vi"><small>D</small>-xylose dehydrogenase xylB                    
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                    <a href="/Part:BBa_K1602009">(BBa_K1602009)</a></li>
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                    <li class="block-10vi"><small>D</small>-xylonolactone lactonase   
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xylC                    
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                    <a href="/Part:BBa_K1602010">(BBa_K1602010)</a></li>
 +
 
 
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                         <img style="width: 332px; height: 200px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7e/T7_gre3_operon_v1.png" align="center">  
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                         <img style="width: 332px; height: 200px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7e/T7_gre3_ operon_v1.png" align="center">  
 
                  
 
                  
 
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https://static.igem.org/mediawiki/2015/a/a1/Itaconic_acid_gene_operon.png"> </div>  
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https://static.igem.org/mediawiki/2015/a/a1/Itaconic_acid_gene_ operon.png"> </div>  
 
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                     <b>Figure 2</b> Genetic map of the xylose to xylitol converting operon with T7 promoter. This brick enables <i>E.Coli</i> BL21 cells to convert xylose to xylitol in presence of the inductor IPTG.
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                     <b>Figure 2</b>  
 
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===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
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<partinfo>BBa_K1602005 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1602017 SequenceAndFeatures</partinfo>

Revision as of 17:26, 17 September 2015

D-xylonic acid producing operon

D-Xylose is a monosaccharide belonging to the aldopentose family. It was recently shown that the D-xylose dehydrogenase xylB from Caulobacter crescentus can convert D-xylose to D-xylonolactone. This can react spontaneously or through the catalysation of xylC to D-xylonic acid. In E. coli D-xylonic acid can be further metabolized to ethyleneglycol.


Figure 1



Usage

This part is a composite of two coding genes with strong RBS (BBa_B0034). The transcription is controlled by a T7 promotor (BBa_I719005).


Figure 2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]