Difference between revisions of "Part:BBa K1616005:Experience"

 
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===Applications of BBa_K1616005===
 
===Applications of BBa_K1616005===
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<h2><font style="color:#b22222"> Notebook </font></h2>
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<h2><font style="color:#b22222"> Results </font></h2>
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<center> https://static.igem.org/mediawiki/2015/c/c5/Graph-150915.png </center>
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Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.
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In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:08, 22 September 2015


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Please enter how you used this part and how it worked out.

Applications of BBa_K1616005

Notebook


Results

Graph-150915.png


Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.

In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.

User Reviews

UNIQ823f62ca3a75950b-partinfo-00000000-QINU UNIQ823f62ca3a75950b-partinfo-00000001-QINU