Difference between revisions of "Part:BBa J45992:Experience"
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<partinfo>BBa_J45992</partinfo> was no longer in stock so we submitted it once again. We used it to express our Curdlan synthase gene (crdS) <partinfo>BBa_K1686046 </partinfo> in stationary phase. <partinfo>BBa_J45992</partinfo> worked as expected, activating our gene only in stationary phase. | <partinfo>BBa_J45992</partinfo> was no longer in stock so we submitted it once again. We used it to express our Curdlan synthase gene (crdS) <partinfo>BBa_K1686046 </partinfo> in stationary phase. <partinfo>BBa_J45992</partinfo> worked as expected, activating our gene only in stationary phase. |
Revision as of 07:31, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45992
A stationary phase-dependent PoPS source.
User Reviews
UNIQ3f26d154626aa16a-partinfo-00000000-QINU
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BBa_J45992, when used to regulate transcription of GFP in BBa_J45995 or banana odor enzyme generator in BBa_J45250, demonstrated the expected behavior. BBa_J45992 produced a low transcriptional signal in exponential phase and a high transcriptional signal in stationary phase. |
•••••
Jean Descarpentrie |
BBa_J45992 was no longer in stock so we submitted it once again. We used it to express our Curdlan synthase gene (crdS) BBa_K1686046 in stationary phase. BBa_J45992 worked as expected, activating our gene only in stationary phase. |
UNIQ3f26d154626aa16a-partinfo-0000000A-QINU
Characterization
Transcriptional control of GFP generator
Transcriptional control of banana odor enzyme generator
Curdlan synthase under the expression of stationary phase promoter
To demonstrate growth phase dependent activation of our Curdlan synthase gene, we compared the amount of Curdlan produced by E.coli while at the same time looking at OD to measure growth. The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.