Difference between revisions of "Part:BBa K1796011"
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<partinfo>BBa_K1796011 short</partinfo> | <partinfo>BBa_K1796011 short</partinfo> | ||
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<partinfo>BBa_K1796011 parameters</partinfo> | <partinfo>BBa_K1796011 parameters</partinfo> | ||
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| + | ==Parameter of Protein== | ||
| + | <p> Number of amino acids: 475 </p> | ||
| + | <p> </p> | ||
| + | <p> Molecular weight: 51557.1 </p> | ||
| + | <p> </p> | ||
| + | <p> Theoretical pI: 8.13 </p> | ||
| + | <p> </p> | ||
| + | <p> Amino acid composition: </p> | ||
| + | <p> </p> | ||
| + | <p> Ala (A) 46 9.7% </p> | ||
| + | <p> </p> | ||
| + | <p> Arg (R) 31 6.5% </p> | ||
| + | <p> </p> | ||
| + | <p> Asn (N) 10 2.1% </p> | ||
| + | <p> </p> | ||
| + | <p> Asp (D) 22 4.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Cys (C) 8 1.7% </p> | ||
| + | <p> </p> | ||
| + | <p> Gln (Q) 14 2.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Glu (E) 25 5.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Gly (G) 42 8.8% </p> | ||
| + | <p> </p> | ||
| + | <p> His (H) 15 3.2% </p> | ||
| + | <p> </p> | ||
| + | <p> Ile (I) 21 4.4% </p> | ||
| + | <p> </p> | ||
| + | <p> Leu (L) 56 11.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Lys (K) 18 3.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Met (M) 13 2.7% </p> | ||
| + | <p> </p> | ||
| + | <p> Phe (F) 15 3.2% </p> | ||
| + | <p> </p> | ||
| + | <p> Pro (P) 24 5.1% </p> | ||
| + | <p> </p> | ||
| + | <p> Ser (S) 44 9.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Thr (T) 24 5.1% </p> | ||
| + | <p> </p> | ||
| + | <p> Trp (W) 8 1.7% </p> | ||
| + | <p> </p> | ||
| + | <p> Tyr (Y) 9 1.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Val (V) 30 6.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Pyl (O) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Sec (U) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (B) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (Z) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (X) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Total number of negatively charged residues (Asp + Glu): 47 </p> | ||
| + | <p> Total number of positively charged residues (Arg + Lys): 49 </p> | ||
| + | <p> </p> | ||
| + | <p> Atomic composition:Carbon C 2282 </p> | ||
| + | <p> Hydrogen H 3634 </p> | ||
| + | <p> Nitrogen N 648 </p> | ||
| + | <p> Oxygen O 671 </p> | ||
| + | <p> Sulfur S 21 </p> | ||
| + | <p> </p> | ||
| + | <p> Formula: C2282H3634N648O671S21Total number of atoms: 7256 </p> | ||
| + | <p> </p> | ||
| + | <p> Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 57910 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 1.123, assuming all pairs of Cys residues form cystines </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 57410 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 1.114, assuming all Cys residues are reduced </p> | ||
| + | <p> </p> | ||
| + | <p> Estimated half-life:The N-terminal of the sequence considered is G (Gly). </p> | ||
| + | <p> </p> | ||
| + | <p> The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). </p> | ||
| + | <p> >20 hours (yeast, in vivo). </p> | ||
| + | <p> >10 hours (Escherichia coli, in vivo). </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Instability index:The instability index (II) is computed to be 40.14 </p> | ||
| + | <p> This classifies the protein as unstable. </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Aliphatic index: 91.22 </p> | ||
| + | <p> </p> | ||
| + | <p> Grand average of hydropathicity (GRAVY): -0.062 </p> | ||
Revision as of 21:31, 18 September 2015
nifN promoted from Paenibacillus sp. WLY78
Function: scaffold gene of nitrogen fixation system
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961
Illegal NgoMIV site found at 648
Illegal AgeI site found at 25
Illegal AgeI site found at 147
Illegal AgeI site found at 766
Illegal AgeI site found at 987 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 475
Molecular weight: 51557.1
Theoretical pI: 8.13
Amino acid composition:
Ala (A) 46 9.7%
Arg (R) 31 6.5%
Asn (N) 10 2.1%
Asp (D) 22 4.6%
Cys (C) 8 1.7%
Gln (Q) 14 2.9%
Glu (E) 25 5.3%
Gly (G) 42 8.8%
His (H) 15 3.2%
Ile (I) 21 4.4%
Leu (L) 56 11.8%
Lys (K) 18 3.8%
Met (M) 13 2.7%
Phe (F) 15 3.2%
Pro (P) 24 5.1%
Ser (S) 44 9.3%
Thr (T) 24 5.1%
Trp (W) 8 1.7%
Tyr (Y) 9 1.9%
Val (V) 30 6.3%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:Carbon C 2282
Hydrogen H 3634
Nitrogen N 648
Oxygen O 671
Sulfur S 21
Formula: C2282H3634N648O671S21Total number of atoms: 7256
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 57910
Abs 0.1% (=1 g/l) 1.123, assuming all pairs of Cys residues form cystines
Ext. coefficient 57410
Abs 0.1% (=1 g/l) 1.114, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 40.14
This classifies the protein as unstable.
Aliphatic index: 91.22
Grand average of hydropathicity (GRAVY): -0.062