Difference between revisions of "Part:BBa K1796010"

 
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__NOTOC__
 
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<partinfo>BBa_K1796010 short</partinfo>
 
<partinfo>BBa_K1796010 short</partinfo>
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<partinfo>BBa_K1796010 parameters</partinfo>
 
<partinfo>BBa_K1796010 parameters</partinfo>
 
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 +
 +
==Parameter of Protein==
 +
<p> Number of amino acids: 1386 </p>
 +
<p> </p>
 +
<p> Molecular weight: 113485.5 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 4.98 </p>
 +
<p> </p>
 +
<p> Amino acid composition: </p>
 +
<p> </p>
 +
<p> Ala (A) 349      25.2% </p>
 +
<p> </p>
 +
<p> Arg (R)  0      0.0% </p>
 +
<p> </p>
 +
<p> Asn (N)  0      0.0% </p>
 +
<p> </p>
 +
<p> Asp (D)  0      0.0% </p>
 +
<p> </p>
 +
<p> Cys (C) 338      24.4% </p>
 +
<p> </p>
 +
<p> Gln (Q)  0      0.0% </p>
 +
<p> </p>
 +
<p> Glu (E)  0      0.0% </p>
 +
<p> </p>
 +
<p> Gly (G) 383      27.6% </p>
 +
<p> </p>
 +
<p> His (H)  0      0.0% </p>
 +
<p> </p>
 +
<p> Ile (I)  0      0.0% </p>
 +
<p> </p>
 +
<p> Leu (L)  0      0.0% </p>
 +
<p> </p>
 +
<p> Lys (K)  0      0.0% </p>
 +
<p> </p>
 +
<p> Met (M)  0      0.0% </p>
 +
<p> </p>
 +
<p> Phe (F)  0      0.0% </p>
 +
<p> </p>
 +
<p> Pro (P)  0      0.0% </p>
 +
<p> </p>
 +
<p> Ser (S)  0      0.0% </p>
 +
<p> </p>
 +
<p> Thr (T) 316      22.8% </p>
 +
<p> </p>
 +
<p> Trp (W)  0      0.0% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  0      0.0% </p>
 +
<p> </p>
 +
<p> Val (V)  0      0.0% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 0 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 0 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              4091 </p>
 +
<p> Hydrogen    H        6798 </p>
 +
<p> Nitrogen    N        1386 </p>
 +
<p> Oxygen      O        1703 </p>
 +
<p> Sulfur      S          338 </p>
 +
<p> </p>
 +
<p> Formula: C4091H6798N1386O1703S338Total number of atoms: 14316 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p>
 +
<p> this could result in more than 10% error in the computed extinction coefficient. </p>
 +
<p> </p>
 +
<p> Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient    21125 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.186, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient        0 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.000, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is C (Cys). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             >20 hours (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 42.28 </p>
 +
<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 25.18 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): 0.793 </p>

Revision as of 21:26, 18 September 2015

nifE promoted from Paenibacillus sp. WLY78

Function: scaffold


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 1386

Molecular weight: 113485.5

Theoretical pI: 4.98

Amino acid composition:

Ala (A) 349 25.2%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 338 24.4%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 383 27.6%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 316 22.8%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4091

Hydrogen H 6798

Nitrogen N 1386

Oxygen O 1703

Sulfur S 338

Formula: C4091H6798N1386O1703S338Total number of atoms: 14316

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 21125

Abs 0.1% (=1 g/l) 0.186, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is C (Cys).

The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 42.28

This classifies the protein as unstable.

Aliphatic index: 25.18

Grand average of hydropathicity (GRAVY): 0.793