Difference between revisions of "Part:BBa K1680018"
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<partinfo>BBa_K1680018 short</partinfo> | <partinfo>BBa_K1680018 short</partinfo> | ||
− | Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of | + | Protein coding region for a fusion construct of Cre Recombinase (BBa_K1680007) and 145N Dronpa (BBa_K1680006) with intermediate 9 amino acid linker (BBa_K1680000). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light. Part is cloned in the pTUM104 (BBa_K801004) and thereby galactose inducible. |
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Revision as of 01:05, 26 September 2015
Cre-Dronpa fusion
Protein coding region for a fusion construct of Cre Recombinase (BBa_K1680007) and 145N Dronpa (BBa_K1680006) with intermediate 9 amino acid linker (BBa_K1680000). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light. Part is cloned in the pTUM104 (BBa_K801004) and thereby galactose inducible.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1345
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 458