Difference between revisions of "Part:BBa K1796007"

 
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__NOTOC__
 
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<partinfo>BBa_K1796007 short</partinfo>
 
<partinfo>BBa_K1796007 short</partinfo>
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<partinfo>BBa_K1796007 parameters</partinfo>
 
<partinfo>BBa_K1796007 parameters</partinfo>
 
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 +
 +
==Parametre of Protein==
 +
<p> Number of amino acids: 1524 </p>
 +
<p> </p>
 +
<p> Molecular weight: 124814.2 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 4.97 </p>
 +
<p> </p>
 +
<p> Amino acid composition: </p>
 +
<p> </p>
 +
<p> Ala (A) 403      26.4% </p>
 +
<p> </p>
 +
<p> Arg (R)  0      0.0% </p>
 +
<p> </p>
 +
<p> Asn (N)  0      0.0% </p>
 +
<p> </p>
 +
<p> Asp (D)  0      0.0% </p>
 +
<p> </p>
 +
<p> Cys (C) 365      24.0% </p>
 +
<p> </p>
 +
<p> Gln (Q)  0      0.0% </p>
 +
<p> </p>
 +
<p> Glu (E)  0      0.0% </p>
 +
<p> </p>
 +
<p> Gly (G) 407      26.7% </p>
 +
<p> </p>
 +
<p> His (H)  0      0.0% </p>
 +
<p> </p>
 +
<p> Ile (I)  0      0.0% </p>
 +
<p> </p>
 +
<p> Leu (L)  0      0.0% </p>
 +
<p> </p>
 +
<p> Lys (K)  0      0.0% </p>
 +
<p> </p>
 +
<p> Met (M)  0      0.0% </p>
 +
<p> </p>
 +
<p> Phe (F)  0      0.0% </p>
 +
<p> </p>
 +
<p> Pro (P)  0      0.0% </p>
 +
<p> </p>
 +
<p> Ser (S)  0      0.0% </p>
 +
<p> </p>
 +
<p> Thr (T) 349      22.9% </p>
 +
<p> </p>
 +
<p> Trp (W)  0      0.0% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  0      0.0% </p>
 +
<p> </p>
 +
<p> Val (V)  0      0.0% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 0 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 0 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              4514 </p>
 +
<p> Hydrogen    H        7506 </p>
 +
<p> Nitrogen    N        1524 </p>
 +
<p> Oxygen      O        1874 </p>
 +
<p> Sulfur      S          365 </p>
 +
<p> </p>
 +
<p> Formula: C4514H7506N1524O1874S365Total number of atoms: 15783 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p>
 +
<p> this could result in more than 10% error in the computed extinction coefficient. </p>
 +
<p> </p>
 +
<p> Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient    22750 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.182, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient        0 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.000, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is A (Ala). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             >20 hours (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 39.16 </p>
 +
<p> This classifies the protein as stable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 26.44 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): 0.808 </p>

Revision as of 21:00, 18 September 2015

nifB from Paenibacillus sp. WLY78

essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1156
    Illegal AgeI site found at 129
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1261


Parametre of Protein

Number of amino acids: 1524

Molecular weight: 124814.2

Theoretical pI: 4.97

Amino acid composition:

Ala (A) 403 26.4%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 365 24.0%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 407 26.7%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 349 22.9%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4514

Hydrogen H 7506

Nitrogen N 1524

Oxygen O 1874

Sulfur S 365

Formula: C4514H7506N1524O1874S365Total number of atoms: 15783

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 22750

Abs 0.1% (=1 g/l) 0.182, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 39.16

This classifies the protein as stable.

Aliphatic index: 26.44

Grand average of hydropathicity (GRAVY): 0.808