Difference between revisions of "Part:BBa K1632022"

Revision as of 12:37, 17 September 2015

J23100_rbs_lasR_TT_Plux_rbs_CmRssrA

At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_lasR_TT_Plux_rbs_cmR to characterize the function of rbs_cmR(BBa_K395160 by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm)

Fig. 1. The cells growth with Cm

From the results of this experiment, initially designed circuits showed leaky expression of CmR. So we inserted an ssrA degradation tag to the C-terminal of CmR.

For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 383
Illegal AgeI site found at 580
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 927

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1632022 short</partinfo>
+At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_lasR_TT_Plux_rbs_cmR to characterize the function of rbs_cmR(<partinfo>BBa_K395160</partinfo> by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm)
+[[Image:Tokyo_Tech Pcon_rbs_lasR_TT_Plux_rbs_cmR.png|thumb|center|550px|<b>Fig. 1.</b> The cells growth with Cm]]
+From the results of this experiment, initially designed circuits showed leaky expression of CmR.  So we inserted an ssrA degradation tag to the C-terminal of CmR.
 For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].