Difference between revisions of "Part:BBa K1632020"
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− | At the first stage of wet experiment, we used the cells which have the plasmid which is | + | At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_rhlR_TT_Plux_rbs_cmR to characterize the function of rbs_cmR(<partinfo>BBa_K395160</partinfo> by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm) |
[[Image:Tokyo_Tech Pcon_rbs_rhlR_TT_Plux_rbs_cmR.png|thumb|center|550px|<b>Fig. 1.</b> The cells growth with Cm]] | [[Image:Tokyo_Tech Pcon_rbs_rhlR_TT_Plux_rbs_cmR.png|thumb|center|550px|<b>Fig. 1.</b> The cells growth with Cm]] |
Revision as of 12:39, 17 September 2015
rbs_CmR(ssrA degradation tag)
At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_rhlR_TT_Plux_rbs_cmR to characterize the function of rbs_cmR(BBa_K395160 by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm)
From the results of this experiment, initially designed circuits showed leaky expression of CmR. So we inserted an ssrA degradation tag to the C-terminal of CmR.
For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]