Difference between revisions of "Part:BBa K1769000:Experience"
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<p>We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.</p> | <p>We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.</p> | ||
<p>The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10<sup>-6</sup>M wortmannin, dimeric FYVE redistributed in cytoplasm.</p> <br> | <p>The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10<sup>-6</sup>M wortmannin, dimeric FYVE redistributed in cytoplasm.</p> <br> | ||
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<figure> | <figure> | ||
<img src="https://static.igem.org/mediawiki/parts/7/75/Nymu-fyve.jpg" style="padding-left:15%;max-width:40%;"> | <img src="https://static.igem.org/mediawiki/parts/7/75/Nymu-fyve.jpg" style="padding-left:15%;max-width:40%;"> | ||
<figcaption>Fig123</figcaption> | <figcaption>Fig123</figcaption> | ||
| − | <img src="https://static.igem.org/mediawiki/parts/1/1b/Nymu-fyve-wort.jpg" style="max-width:40%;"><figcaption>Fig123</figcaption></figure></html> | + | </figure> |
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| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/1/1b/Nymu-fyve-wort.jpg" style="max-width:40%;"><figcaption>Fig123</figcaption></figure> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:36, 17 September 2015
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1769000
Results
We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.
The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10-6M wortmannin, dimeric FYVE redistributed in cytoplasm.
User Reviews
UNIQe641332fb7f268a7-partinfo-00000001-QINU UNIQe641332fb7f268a7-partinfo-00000002-QINU