Difference between revisions of "Part:BBa K1847015"
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1847015 SequenceAndFeatures</partinfo>
  
 
Fusion between the ice nucleation protein (INP) and annexing V. RFP is found in the sequence to facilitate localization of plasmids.   
 
Fusion between the ice nucleation protein (INP) and annexing V. RFP is found in the sequence to facilitate localization of plasmids.   
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[[File:ETH15_Only_INP_annexin_gel.jpg|right|500px|thumb|Figure 1. INP-Annexin V expression.]]
 
[[File:ETH15_Only_INP_annexin_gel.jpg|right|500px|thumb|Figure 1. INP-Annexin V expression.]]
 
However, we were not able to demonstrate externalization of INP in the membrane.  
 
However, we were not able to demonstrate externalization of INP in the membrane.  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1847015 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 10:05, 17 September 2015

promoter-RBS-INP-annexin V-RFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2075
    Illegal NheI site found at 2098
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal AgeI site found at 2684
    Illegal AgeI site found at 2796
  • 1000
    COMPATIBLE WITH RFC[1000]

Fusion between the ice nucleation protein (INP) and annexing V. RFP is found in the sequence to facilitate localization of plasmids. Part made using the INP submitted by the Edinburg team 2011 Part:BBa_K523013.

INP-Annexin V is expressed in the membrane fraction of the cell, as it is shown in Figure 1.

Figure 1. INP-Annexin V expression.

However, we were not able to demonstrate externalization of INP in the membrane.