Difference between revisions of "Part:BBa K1820014:Experience"

(Applications of BBa_K1820014)
 
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We created and tested this construct in ''Escherichia coli'' in the pSB1C3 plasmid. It was tested for fluorescence relative to non-transformed ''E. coli'' cell and two other similar constructs (see Figure 1).
 
We created and tested this construct in ''Escherichia coli'' in the pSB1C3 plasmid. It was tested for fluorescence relative to non-transformed ''E. coli'' cell and two other similar constructs (see Figure 1).
  
<figure style="text-align:center">
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  <img src=https://static.igem.org/mediawiki/parts/5/5d/Utah_State2015_SfGFP%28Bs%29_Fluorescence_Chart.jpg width="479" height="286" alt="0"/>
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https://static.igem.org/mediawiki/parts/5/5d/Utah_State2015_SfGFP%28Bs%29_Fluorescence_Chart.jpg
  <figcaption><strong> Figure 1. </strong> Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in ''E. coli'' excited at 485/20 with emissions read at 528/20. </figcaption>
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</figure>
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<p><strong> Figure 1. </strong> Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in ''E. coli'' excited at 485/20 with emissions read at 528/20. </p>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 04:53, 17 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1820014

We created and tested this construct in Escherichia coli in the pSB1C3 plasmid. It was tested for fluorescence relative to non-transformed E. coli cell and two other similar constructs (see Figure 1).


Utah_State2015_SfGFP%28Bs%29_Fluorescence_Chart.jpg

Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 485/20 with emissions read at 528/20.

User Reviews

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