Difference between revisions of "Part:BBa K1732009"

Revision as of 09:12, 18 September 2015

pSB1C3-J23100-B0034-GFP-His-B0015

His tagged GFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. GFP was measured at (ex/em = 488nm/509nm).

Sequence alignment of original sequence to codon optimized sequence, indicating the differences in amino acids.

The graphs below show the data for the original set of fluorescent proteins prior to being codon optimized via the IDT codon optimization tool. The set consists of His tagged RFP, OFP, YFP, eGFP, and BFP, each with a strong RBS and WT lac promoter that allows for Lac induction of fluorescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 740
Illegal AgeI site found at 209
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 [[File:FQuantitation.jpg]]
+----
+Sequence alignment of original sequence to codon optimized sequence, indicating the differences in amino acids.
+[[File:Alignment GFP.jpg]]
+----
+The graphs below show the data for the original set of fluorescent proteins prior to being codon optimized via the IDT codon optimization tool. The set consists of His tagged RFP, OFP, YFP, eGFP, and BFP, each with a strong RBS and WT lac promoter that allows for Lac induction of fluorescence.
+[[File:Fluorescent_proteins_characterization.jpg]]
+[[File:Fluorescent_proteins_Amounts.jpg]]
 <!-- Add more about the biology of this part here