Difference between revisions of "Part:BBa K1859015"
| Line 28: | Line 28: | ||
</p> | </p> | ||
| − | <p> | + | <p> |
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > BBa_K1859016 </a> | + | You can let mRNA circularize efficiently by using BBa_K1859015 and |
| − | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > BBa_K1859016 </a> | |
| − | + | than using parts of 2014.</p> | |
| − | + | ||
</html> | </html> | ||
Revision as of 04:50, 17 September 2015
the 3´side of the intron[BBa_K1332005] +complementary seqence
BBa_K1859015 can let mRNA circularize efficiently in E. coli by combining it with BBa_K1859016 .
As for details of circular mRNA, refer to ‘iGEM Gifu 2014' .
We improved the 5´side of the intron [BBa_K1332003] in iGEM2014 and made BBa_K1859015. Circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , in 2014 cloned only sequence of ribozyme involved splicing from td intron in T4 phage. However, BBa_K1859015 is cloned extra a part of intron adding the part in 2014. A part of intron involved in BBa_K1859015 has complementarity with a part of intron involved in BBa_K1859016.
You can let mRNA circularize efficiently by using BBa_K1859015 and BBa_K1859016 than using parts of 2014.