Difference between revisions of "Part:BBa K1859015"
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332003" > [BBa_K1332003] | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332003" > [BBa_K1332003] | ||
</a> in iGEM2014 and made BBa_K1859015. | </a> in iGEM2014 and made BBa_K1859015. | ||
| − | Circular parts,the 3'side of the intron | + | Circular parts, the 3'side of the intron |
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a> | ||
and the 5'side of the intron | and the 5'side of the intron | ||
Revision as of 04:24, 17 September 2015
the 3´side of the intron[BBa_K1332005] +complementary seqence
BBa_K1859015 can let mRNA circularize efficiently in E. coli by combining it with BBa_K1859016 .
As for details of circular mRNA, refer to ‘iGEM Gifu 2014' .
We improved the 5´side of the intron [BBa_K1332003] in iGEM2014 and made BBa_K1859015. Circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , in 2014 cloned only sequence of ribozyme involved splicing from td intron in T4 phage. However, BBa_K1859015 is cloned extra a part of intron adding the part in 2014. A part of intron involved in BBa_K1859015 has complementarity with a part of intron involved in BBa_K1859016.
When circular mRNA is produced using BBa_K1859015 and BBa_K1859016 , efficiency of circularising rises than parts of 2014.