Difference between revisions of "Part:BBa K1689014"

 
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<partinfo>BBa_K1689014 short</partinfo>
 
<partinfo>BBa_K1689014 short</partinfo>
  
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pSB1C3-6H-dCas9-Clact, C terminal segment of beta-lacatamse fused with dCas9, His tag allows for protein purification
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pSB1C3-6His-dCas9-Clact, C terminal segment of beta-lacatamse(Clact) fused with dCas9, 6×His tag allows for protein purification. β-Lactamase catalyzes the hydrolysis of penicillin to penicillinoic acid, causing current changes which can be detected using A3 electrode system. β-Lactamase could be dissected between Gly196(N termina, Nlactl) and Leu198(C terminal,Clact).
  
 
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Revision as of 02:52, 18 September 2015

dCas9-Clact

pSB1C3-6His-dCas9-Clact, C terminal segment of beta-lacatamse(Clact) fused with dCas9, 6×His tag allows for protein purification. β-Lactamase catalyzes the hydrolysis of penicillin to penicillinoic acid, causing current changes which can be detected using A3 electrode system. β-Lactamase could be dissected between Gly196(N termina, Nlactl) and Leu198(C terminal,Clact).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]