Difference between revisions of "Part:BBa K1689011"

Revision as of 16:50, 18 September 2015

dCas9-delta alpha

dCas9 fused with Δα segment of lacz, His tag allows for protein purification

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1150
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal BamHI site found at 3429
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1689011 short</partinfo>
-delta alpha segment of lacz fused with dCas9, His tag allows for protein purification
+dCas9 fused with Δα segment of lacz, His tag allows for protein purification
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+For their ease of use, low assay time, cost-effectiveness, high sensitivity, electrochemical biosensors have emerged as an attractive method for molecular detection.  Combing with our PC Reporter we may further enhance it's applicability, that is to implement point-of-care diagnostics in settings outside clinics and without the need of  specialized analytical laboratories.
+β-glactosidase (LacZ) is an exoglycosidase from E.coli,  composing of 1024 amino acids. LacZ is a commonly used as a reporter, known for blue white screen, which catalyzes X-gal to produce blue dye; by using different substrates, for example ONPG, reaction can be measured quantitatively.
+In our project, we could dissect LacZ as Δα, ∆ω segments to fuse with dCas9 to do the same as our PC Reporter.
+β-galactosidase is used to catalyze the hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG) to p-aminophenol (PAP), which can be oxidized at an working electrode held at 220mV versus the reference electrode.
 <!-- -->