Difference between revisions of "Part:BBa K1689017:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1689017 short</partinfo>
 
<partinfo>BBa_K1689017 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The coding sequence of this fusion protein is too long, you'd better amplify is separately.
+
The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
  
  
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
E.coli
+
''E.coli''
  
 
===References===
 
===References===

Latest revision as of 15:51, 27 September 2015

F3-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3672
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.


Source

E.coli

References