Difference between revisions of "Part:BBa K1871010"
(→‎Alpha Amylase)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K1871000 short</partinfo>
+
<partinfo>BBa_K1871010 short</partinfo>
  
 
[[File:alphaamylase.jpg|200 px|right|Alpha amylase protein]]
 
[[File:alphaamylase.jpg|200 px|right|Alpha amylase protein]]
Line 14: Line 14:
  
 
====Alpha Amylase====
 
====Alpha Amylase====
−
Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.
+
Obtained from ''Bacillus amyloliquefaciens'', α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.
  
 
====ADH1 Terminator====
 
====ADH1 Terminator====
Line 25: Line 25:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K1871000 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1871010 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K1871000 parameters</partinfo>
+
<partinfo>BBa_K1871010 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 00:56, 17 September 2015

Coding Sequence AMY1

Alpha amylase protein


This is an alpha amylase gene obtained from Bacillus amyloliquefaciens. It is flanked by a Kozak sequence and ADH1 terminator but lacks a promoter.

Kozak Sequence

This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

Native Alpha Amylase Secretion Sequence

This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1436
    Illegal NgoMIV site found at 1451
  • 1000
    COMPATIBLE WITH RFC[1000]