Difference between revisions of "Part:BBa K1602030"
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+ | This part codes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface. | ||
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Latest revision as of 01:25, 19 September 2015
HisTag-Scaffold_Si4-Tag fusion protein
This part codes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 22
Illegal BamHI site found at 877 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 94
Illegal AgeI site found at 217 - 1000COMPATIBLE WITH RFC[1000]