Difference between revisions of "Part:BBa K1871010"
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| − | This is an alpha amylase gene obtained from ''Bacillus amyloliquefaciens''. It is flanked by a Kozak sequence and ADH1 terminator. | + | This is an alpha amylase gene obtained from ''Bacillus amyloliquefaciens''. It is flanked by a Kozak sequence and ADH1 terminator but lacks a promoter. |
====Kozak Sequence==== | ====Kozak Sequence==== | ||
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====Native Alpha Amylase Secretion Sequence==== | ====Native Alpha Amylase Secretion Sequence==== | ||
| − | + | This is the ''Bacillus amyloliquefaciens'' alpha amylase signal sequence, which directs the protein to be secreted. | |
====Alpha Amylase==== | ====Alpha Amylase==== | ||
Revision as of 00:55, 17 September 2015
No part name specified with partinfo tag.
This is an alpha amylase gene obtained from Bacillus amyloliquefaciens. It is flanked by a Kozak sequence and ADH1 terminator but lacks a promoter.
Kozak Sequence
This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.
Native Alpha Amylase Secretion Sequence
This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.
Alpha Amylase
Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.
ADH1 Terminator
This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.
Sequence and Features
No part name specified with partinfo tag.