Difference between revisions of "Part:BBa K1678002"

Latest revision as of 11:55, 21 September 2015

RibT promoted by p25 synthetic promoter

Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.
Coding sequence has been codon optimized for Lactobacillus plantarum.
Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019).
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
The expressed protein catalyses two steps in the riboflavin pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 22
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 553

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1678002 short</partinfo>
-Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.
+Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase. <br>
-Coding sequence has been codon optimized for Lactobacillus plantarum.
+Coding sequence has been codon optimized for Lactobacillus plantarum. <br>
-Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019).
+Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019). <br>
-The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
+The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).<br>
-The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
+The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). <br>
-The expressed protein catalyses two steps in the riboflavin pathway.
+The expressed protein catalyses two steps in the riboflavin pathway.<br>
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