Difference between revisions of "Part:BBa K1678001:Design"
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Latest revision as of 22:32, 16 September 2015
Pyrimidine reductase/pyrimidine deaminase cassette RibD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1262
Design Notes
RibD is not considered as a bottleneck in the riboflavin pathway. A medium promoter is so expressing it.
Source
Coding sequence comes from Bacillus subtilis genome sequence. It has been codon optimized for Lactobaciilus plantarum on IDT website. p25 is a synthetic constitutive promoter designed in 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006. The RBS informations are coming from Tauer et al. Microbial Cell Factories 2014, 13:150. Tldh terminator information are documented in Spath et al. Microbial Cell Factories 2012, 11:141.