Difference between revisions of "Part:BBa K1820026"

 
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<partinfo>BBa_K1820026 short</partinfo>
 
<partinfo>BBa_K1820026 short</partinfo>
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This part is a reporter sequence designed to produce superfolder green fluorescent protein when the host cell is infected by the P335 species lactococcal phage Φ31.
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===Usage and Biology===
 
===Usage and Biology===
  
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This construct is built from a promoter from the P335 species lactococcal phage Φ31, a native ribosome binding site, a sfGFP that is known to work well in ''Lactococcus lactis'', and a double stop terminator. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 02:05, 19 September 2015

p2001-native RBS-sfGFP-B0015

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This part is a reporter sequence designed to produce superfolder green fluorescent protein when the host cell is infected by the P335 species lactococcal phage Φ31.

Usage and Biology

This construct is built from a promoter from the P335 species lactococcal phage Φ31, a native ribosome binding site, a sfGFP that is known to work well in Lactococcus lactis, and a double stop terminator. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 333