Difference between revisions of "Part:BBa K1725000"
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<partinfo>BBa_K1725000 short</partinfo>
 
<partinfo>BBa_K1725000 short</partinfo>
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This promoter was characterised by driving expression of GFP with two different Ribosome Binding Sites, as found in the BioBricks E5501 and I13500.
 
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GFP fluorescence of K1725001, K1725002, K1725021, K1725022, K1725082, and E5504 with plasmid backbone pSB3K3 was measured to compare the relative strengths of promoters K1725000 and K1725020 to a promoter already well documented in the registry, R0040. Figure 3 shows the fluorescence scan image and a graph of approximate molecules of GFP per cell. These results indicated that K1725000 is a significantly stronger promoter than R0040 or K1725020. It also further confirms that B0034 is a stronger ribosome binding site that B0032.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1725000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1725000 SequenceAndFeatures</partinfo>
 +
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This promoter was characterised by driving expression of GFP with two different Ribosome Binding Sites, as found in the BioBricks E5501 and I13500.
 +
 +
GFP fluorescence of K1725001, K1725002, K1725021, K1725022, K1725082, and E5504 with plasmid backbone pSB3K3 was measured to compare the relative strengths of promoters K1725000 and K1725020 to a promoter already well documented in the registry, R0040. Figure 3 shows the fluorescence scan image and a graph of approximate molecules of GFP per cell. These results indicated that K1725000 is a significantly stronger promoter than R0040 or K1725020. It also further confirms that B0034 is a stronger ribosome binding site that B0032.
 +
 +
https://static.igem.org/mediawiki/2015/d/df/Glasgow_2015_Repressors_Promoter_Graph_2.png
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 +
<b>All constructs with pSB3K3 plasmid backbone, in DH5α cells. Replicates of constructs and controls from three colonies, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.</b>
  
  

Revision as of 19:57, 16 September 2015

PhlF repressible promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This promoter was characterised by driving expression of GFP with two different Ribosome Binding Sites, as found in the BioBricks E5501 and I13500.

GFP fluorescence of K1725001, K1725002, K1725021, K1725022, K1725082, and E5504 with plasmid backbone pSB3K3 was measured to compare the relative strengths of promoters K1725000 and K1725020 to a promoter already well documented in the registry, R0040. Figure 3 shows the fluorescence scan image and a graph of approximate molecules of GFP per cell. These results indicated that K1725000 is a significantly stronger promoter than R0040 or K1725020. It also further confirms that B0034 is a stronger ribosome binding site that B0032.

Glasgow_2015_Repressors_Promoter_Graph_2.png

All constructs with pSB3K3 plasmid backbone, in DH5α cells. Replicates of constructs and controls from three colonies, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.