Difference between revisions of "Part:BBa K653000:Experience"

(→‎User Reviews)
(→‎Applications of BBa_K653000)
Line 4: Line 4:
  
 
===Applications of BBa_K653000===
 
===Applications of BBa_K653000===
 +
<html>
 +
<body width="80%" padding-left="4%">
 +
<div style="text-align:justify">
 +
<h4>Experience of the ITB_Indonesia iGEM 2015 team.</h4>
 +
 +
The iGEM 2015 ITB Indonesia used this coding sequence under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in <i>E. coli</i> BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity. <br>
 +
Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.<br>
 +
This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.
 +
 +
</div>
 +
</body>
 +
</html>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:05, 16 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K653000

Experience of the ITB_Indonesia iGEM 2015 team.

The iGEM 2015 ITB Indonesia used this coding sequence under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in E. coli BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity.
Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.
This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.

User Reviews

UNIQ95cdd014d3f2c5e3-partinfo-00000001-QINU UNIQ95cdd014d3f2c5e3-partinfo-00000002-QINU