Difference between revisions of "Part:BBa K1597000:Experience"
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We have cloned this promoter out of the genome of Bacillus subtilis 168 and combined it with tasA gene.
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We have cloned this promoter out of the genome of ''Bacillus subtili''s 168 and combined it with the tasA gene (produces amyloid-like fibers) from ''B. subtilis''.
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Both B. subtilis ComI strain with salt promoter and tasA construct (TASA) and  B. subtilis ComI  (COMI) were grown on Msgg media with and without salt. After 24 hours thioflavin S (which is an amyloid fiber staining) was added to the biofilms. the biofilms were photographed with white light and fluorescence after 15 min incubation.
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Both ''B. subtilis'' ComI strain with salt promoter and tasA construct (''TASA'') and  ''B. subtilis'' ComI  (''COMI'') were grown on Msgg media with and without salt. After 24 hours thioflavin S (which is an amyloid fiber staining) was added to the biofilms. the biofilms were photographed with white light and fluorescence after 15 min incubation.
  
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With the induction of salt, TASA shows more amyloid fibers are present after 24 hours.This is not the case for COMI. This means that the salt promoter is activated with salt and produces extra tasA, resulting in a more intense dye.  
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With the induction of salt, ''TASA'' shows more amyloid fibers are present after 24 hours.This is not the case for COMI. This means that the salt promoter is activated with salt and produces extra tasA, resulting in a more intense dye.  
  
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[https://static.igem.org/mediawiki/parts/6/65/TasA_jpeg_voor_site.jpg]
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<img src="https://static.igem.org/mediawiki/parts/6/65/TasA_jpeg_voor_site.jpg">
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Revision as of 17:08, 16 September 2015

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Applications of BBa_K1597000

We have cloned this promoter out of the genome of Bacillus subtilis 168 and combined it with the tasA gene (produces amyloid-like fibers) from B. subtilis. Both B. subtilis ComI strain with salt promoter and tasA construct (TASA) and B. subtilis ComI (COMI) were grown on Msgg media with and without salt. After 24 hours thioflavin S (which is an amyloid fiber staining) was added to the biofilms. the biofilms were photographed with white light and fluorescence after 15 min incubation.

With the induction of salt, TASA shows more amyloid fibers are present after 24 hours.This is not the case for COMI. This means that the salt promoter is activated with salt and produces extra tasA, resulting in a more intense dye.


User Reviews

UNIQ776fc9a9c6bea59e-partinfo-00000001-QINU UNIQ776fc9a9c6bea59e-partinfo-00000002-QINU