Difference between revisions of "Part:BBa K1767009"
 
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Latest revision as of 08:25, 17 September 2015

P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS eyfp ter ter


We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time.

In order to understand our system we started to test the first part of our genetic circuit , to test it we built the first part ( k1767001 ) driving expression of florescence protein .

The here presented BioBrick consists of a constitutive promoter(I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of YFP.

The idea is to retrieve information about the expression of a fluorescent reporter downstream of the pLuxR promoter in absence and increasing concentrations of AHL.

The scheme below explains how this BioBrick works :

Technion HS Israel K1767009.jpg

We performed an experiment to test this part, and here is the result:


Technion HS Israel K1767009 result.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1761