Difference between revisions of "Part:BBa K1696003"

Revision as of 13:27, 16 September 2015

L-lactate producing part1

L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase (ldhA).[1] Similar metabolic pathway has been found in a variety of organisms, to distinguish the differences, we renamed the heterogenous L-lactate dehydrogenase ldhE while homogenous one keeps original.

We choose a strong promoter+ strong RBS to construct L-Lactate part. The NAD+-dependent L-lactate dehydrogenase gene is from Lactobacillus casei.

Fig.1 Design of the part.

But we found the yield was no different between BL21(blank) and BL21+ldhE(experientmental). Shown in Fig2.

Fig2. The lactate yield of BL21 and BL21+ldhE.

Sequence and Features